The demonstration was held during a May Day march in St Petersburg, with a group of around 10 protesters arrested near the Anichkov Bridge in the centre of Russia’s second city.
Images shared on Twitter showed activists lying on the road pretending to be dead, with fake blood smeared across their faces and covered with rainbow and Chechen flags.
Officers in riot gear were shown arresting protesters and dragging them to a police van.
A separate group of gay-rights protesters were detained with placards that called for the Chechen leader, Ramzan Kadyrov, to be tried at the International Court of Justice in The Hague.
One activist was taken away in an ambulance after he appeared to have fainted.
The action was in protest at the systemic persecution of gay men in the Russian republic of Chechnya.
More than 100 men have been rounded up and detained in secret prisons by authorities in recent weeks, with many beaten and tortured, the Russian newspaper Novaya Gazeta claimed.
At least four are alleged to have been killed.
Ramzan Kadyrov, the President of Chechnya (REUTERS)
Human Rights Watch (HRW) is scathing about the Russian government treatment of LGBT citizens.
The group accuses the Kremlin of “failing in their obligation to prevent and prosecute homophobic violence”.
“Growing numbers of LGBT people have been attacked and harassed across Russia in the lead-up and aftermath of the adoption of the federal anti-LGBT ‘propaganda’ law in June 2013.
“The law effectively legalised discrimination against LGBT people and cast them as second-class citizens.”
In interviews it conducted with gay and lesbian Russians, HRW said LGBT people described being “physically attacked by strangers during their everyday activities”.
The May Day march in St Petersburg was one of dozens around the world.
In many countries, including Turkey, Cambodia, the Philippines and Bangladesh, workers took to the streets to protest about poor conditions and pay.